MCS: 6403..6444
MCS sequence: GGAAGAGCCATGGGCGGCCGCGAATTCCTCGAGGGCTCTTCC
In-frame codons from 6403: 6403:GGA 6406:AGA 6409:GCC 6412:ATG 6415:GGC 6418:GGC 6421:CGC 6424:GAA 6427:TTC 6430:CTC 6433:GAG 6436:GGC 6439:TCT 6442:TCC
In-frame leucine codon(s): 6430-6432 (CTC)
NEBcutter project id: pJT3YYYkWj3-9g1d3_pTWIN2
NEB enzymes with empty dmsen (not methylation-sensitive) whose recognition span covers the in-frame leucine codon:
enzyme	num_sites	recpos	recseq	recsite	ct1	cb1	ct2	cb2
BsoBI	3	6430	CYCGRG	C^TCGA_G	6430	6434	0	0
MnlI	39	6429	CCTC	CCTC	6439	6438	0	0
SmlI	8	6430	CTYRAG	C^TCGA_G	6430	6434	0	0
Subset with a cleavage coordinate inside 6430-6432 (codon bases):
enzyme	num_sites	ct1	cb1	ct2	cb2
BsoBI	3	6430	6434	0	0
SmlI	8	6430	6434	0	0